Chromosomal DNA extraction was carried out using an isolation
kit (Fermentas, Thermo Fisher Scientific, Waltham, MA). Primers
Hdc3F (50AGATGGTATTGTTTCTTATGA30) and Hdc4R (50CAAACACCA
GCATCTTC30) were designed for amplification of a 435 bp length
part of hdc gene (Coton & Coton, 2005).