2.3. Cell culture, transfection and reporter activity assay
TO cells were seeded in 24-well plates (Nunc) at a density of
2 105 cells per well. The cells were transfected using 10 ml Neon®
Transfection System (Invitrogen, Carlsbad, CA) with 10 ml buffer R
and 500 n plasmid in each well, and using 2 pulses of 1100 V and
30 ms each. In each well, cells were transfected with 50 ng of