Due to the enzyme activity, it was realized that total FFAs concentration
(as criterion of oil quality) in untreated sample increased
from 5.6% to 36.0% during 12 weeks. On the other words,
in less than one week from the date of rice milling, the RBO deteriorated
and became inedible. This enzymatic problem effectively
overcame by application of sub-CW treatment. Lipase enzyme
was denatured and as a result, no increase was observed in total
FFAs concentration in the treated RBO by time.