Five hundred ml aqueous crude solution were fractionated
by means of liquid-liquid extraction using four organic solvents,
n-hexane (DC = 2.0), diethyl ether (DC = 4.3), chloroform
(DC = 4.8) and ethyl acetate (DC = 6.0), in order of
increasing polarity and at a ratio of 2ml solvent ml-1 aqueous
crude extract.