Conclusion
In this work, a simple, cost effective and green method was
developed for the determination of carmine in food samples by
using CPE prior to its spectrophotometric detection. The effects
of main parameters such as solution pH, surfactant and salt concentrations,
incubation time and temperature were investigated
and optimized. The results of this study show the potential of the
proposed method for the determination of carmine in food samples.
To the best our knowledge, this is the first report which uses
the cloud point extraction for determination of carmine in real
samples. The method is simple, selective and sensitive for determination
of carmine in food samples and does not require sophisticated
instruments such as high-performance liquid
chromatography or stripping voltammetry. The results were
shown the proposed method can be applied satisfactory to determine
the carmine in food samples.