Rojas-Grau and others (2006) determined the bactericidal effi-ciency against Escherichia coli O157:H7 of apple puree-based FFFs incorporated with oregano (0.1% wt.), lemongrass (0.5% wt.) or cinnamon (0.5% wt.) essential oils and reported BA50 values (that is, the percentage of antimicrobial compound that killed 50% of the tested bacteria under the tested conditions) of 0.034, >0.34, and 0.28, respectively, after incubation for 3 min at 21 °C. Those values were reduced to 0.024, 0.12, and 0.078 in the following 27 min, indicating that oregano essential oil showed the highest bactericidal activity even at the lowest concentration, and that 30 min were required for cinnamon essential oil to be effective. The dried films were also tested against E. coli O157:H7 and, likewise, oregano essential oil showed the best outcome as indicated by the largest inhibition zone around a film disk (Rojas-Grau and ¨ others 2006). In another study, Rojas-Grau and others (2007) in- ¨ corporated apple puree edible films with the same essential oils orwith their major antimicrobial compounds, namely, carvacrol, citral, and cinnamaldehyde, respectively. As expected, the inhibition zones of E. coli O157:H7 created around films incorporated with the antimicrobial compounds were greater than those around films added with the original essential oils, carvacrol having the most pronounced antimicrobial effect. In contrast, the inhibition zones of Staphylococcus aureus and E. coli were larger for carrot puree edible films with cinnamaldehyde than those added with carvacrol (Wang and others 2011a). Carvacrol was also added to apple and tomato puree edible films, which were effective in inhibiting the growth of E. coli O157:H7, with increasing carvacrol contents
Rojas-Grau and others (2006) determined the bactericidal effi-ciency against Escherichia coli O157:H7 of apple puree-based FFFs incorporated with oregano (0.1% wt.), lemongrass (0.5% wt.) or cinnamon (0.5% wt.) essential oils and reported BA50 values (that is, the percentage of antimicrobial compound that killed 50% of the tested bacteria under the tested conditions) of 0.034, >0.34, and 0.28, respectively, after incubation for 3 min at 21 °C. Those values were reduced to 0.024, 0.12, and 0.078 in the following 27 min, indicating that oregano essential oil showed the highest bactericidal activity even at the lowest concentration, and that 30 min were required for cinnamon essential oil to be effective. The dried films were also tested against E. coli O157:H7 and, likewise, oregano essential oil showed the best outcome as indicated by the largest inhibition zone around a film disk (Rojas-Grau and ¨ others 2006). In another study, Rojas-Grau and others (2007) in- ¨ corporated apple puree edible films with the same essential oils or<br>ด้วยสารต้านจุลชีพของพวกเขาที่สำคัญคือ carvacrol, citral และ cinnamaldehyde ตามลำดับ คาดว่าจะเป็นโซนการยับยั้งเชื้อ E. coli O157: H7 สร้างขึ้นรอบหนังรวมกับสารต้านจุลชีพเป็นมากกว่าภาพยนตร์รอบเพิ่มด้วยน้ำมันหอมระเหยเดิม carvacrol ได้เด่นชัดมากที่สุดผลยาต้านจุลชีพ ในทางตรงกันข้ามโซนยับยั้งเชื้อ Staphylococcus aureus และ E. coli มีขนาดใหญ่สำหรับภาพยนตร์กินแครอทซุปข้นกับ cinnamaldehyde กว่าที่เพิ่มเข้ามาด้วย carvacrol (วังและอื่น ๆ 2011a) carvacrol ถูกเพิ่มไปยังแอปเปิ้ลและมะเขือเทศหนังกินซุปข้นซึ่งมีประสิทธิภาพในการยับยั้งการเจริญเติบโตของเชื้อ E. coli O157 นี้: H7 มีการเพิ่มเนื้อหา carvacrol
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