Furthermore, in the first 9 days, no
significant difference in TMC and TPC values was observed between
lot Ch and lot Nch during the refrigerated storage (P > 0.05),
however, on day 12, TMC and TPC of fish treated with Nch were
significantly lower than that treated with Ch (P < 0.05), which could
be due to the higher antimicrobial activity of nanochitosan
compared to chitosan. TMC and TPC of fish treated with Ch and Nch
did not exceed the maximal permissible limit of 7.0 log10 cfu/g until
the end of storage period (except for TPC of lot Ch that reached to
7.18 ± 0.16 log10 cfu/g on day 12), while these values were higher
than 7.0 log10 cfu/g in uncoated (lot C) and acid (lot AC) controls on
days 9 (except for TMC of lot AC) and 12.