Six-week-old multiple shoots derived from the SIM were transferred to a
growing medium based on solid MS medium supplemented with 1 mg/l silver
nitrate and 0.05% activated charcoal but without the addition of a plant growth
regulator to encourage shoot elongation. After 4 weeks of culture, each
elongated shoot was excised and transferred to a half-strength MS medium
supplemented with 5 mg/l IBA, 1 mg/l silver nitrate, 3% sucrose, 0.05%
activated charcoal and 0.75% agar for root induction. Complete plantlets were
then hardened and transferred to a greenhouse