Results
Figure 1 illustrates the effect of glycerol concentration on the
growth of Chlorella sp. in comparison with growth on BG11
medium.
As shown in Fig. 1, a lag phase of growth of
Chlorella sp. was observed for all technical glycerol concentrations.
The sharp increase in concentration was characteristic
of a growth medium with an initial glycerol concentration of
2gL−1 during the exponential growth phase. A slight growth
in biomass is characteristic of a growth medium with initial
concentrations of 5 or 10 g L−1
. Maximum biomass concentrations
of 1.65, 1.92 and 1.70 g L−1 were obtained in the
stationary growth phase in the medium containing 2, 5 and
10 g L−1 glycerol, respectively. In comparison, the highest
concentration achieved using 5 g L−1 of glycerol was 17.7 %
higher than the concentration achieved with Chlorella sp. in
autotrophic medium (BG11 with 120 mg L−1 N), which was
1.58 g L−1