In vivo study
Albino mice were cultured in animal house following Codes
and Commandments of Institutional Ethics Committee
regarding caring, handling and use of animals in the Research
and the work is carried out in accordance with the Code of
Ethics of the World Medical Association (Declaration of
Helsinki). Albino mice 8–10 weeks old were exposed to different
test chemicals by intra peritoneal injection and were sacrificed
at sequential intervals of 16, 24, and 32 h of stipulated
treatment time. Animals were treated with each test substance
as mentioned above. Further processes of slide preparations,
cells and chromosomal aberrations analyses are adopted from
earlier published work [4,5]. The reduction factors due to test
chemical treatments were calculated using the formula
published earlier