Shoots of 2-3 cm in height with two or three leaflets derived from cultures were transferred to MS medium containing 1 mg/l NAA +
20g sucrose or IAA or IBA or 200mg activated charcoal separately for rooting. The rooted shoots were transferred to MS medium for further elongation of the roots. The rooted plants were washed with water to remove media then transferred to pots containing autoclaved vermiculite soil and sand (1:2:1), and covered with polyethylene bags for one week to maintain high humidity and subsequently exposed to low air humidity for increasing period and finally polyethylene bags were removed. These hardened plants then transferred to the greenhouse [6,7].