Puffed shrimp chips were ground with a pulverizer (D3V-10;
Yu Chi Machinery Co., Ltd., Chang Hua, Taiwan), and a 1-g
sample was placed into a 50-mL centrifuge tube. Fifteen milliliters
of deionized distilled water was added, and the solution
was mixed in a 50 C reciprocal shaker bath for 60
minutes.