Changes in TBARS concentration in dried soft tissues of P. canaliculata (Fig. 3A), as indicative of lipid peroxidation, were evaluated by one-way ANOVA followed by the Tukey test. A two- fold and statistically significant increase in animals after 45 days of estivation was observed as compared with the control group of active animals. Also, TBARS significantly dropped to values slightly above the control levels at 20 min and 24 h after operculum detachment. The latter low values, however, were still significantly higher than the active control group.