Soil collection: Soil samples were collected from different gardens, playgrounds and from nearby the laboratory of Lovely Professional University, Punjab, India. Isolation: Serial dilution method was employed for isolation of strains producing antibiotics. Nutrient Agar (NA) was used for screening of bacteria while potato dextrose agar (PDA) was used for fungi. Media and water blanks were prepared and sterilized. Different dilutions (10-3, 10-4 and10-5) and (10-5 and 10-6) were used for fungi and bacteria respectively. Pour plating method was used during the present study. The plates were incubated at 370C for 24 hours for bacterial growth and 270 C for 72 hours for fungal growth. Preservation of isolates: Total twelve soil isolates (No. 01-12) were isolated and these isolates were preserved in the slants of PDA and NA by keeping them into the refrigerator at 40C and maintained for longer period by serial subculturing. In vitro screening of isolates for anti-bacterial activity: Twelve soil isolates were selected for anti-bacterial activity screening against the pathogenic test organisms by