The ANS− probe is usually used to specifically bind to the exposed hydrophobic regions of the protein molecules. The initial slope of the curve plotting FI v.s. protein content reflects the hydrophobic property of the protein, which is called surface hydrophobicity (H0). As shown in Fig 5, the H0 of the blanched soymilk (1277) was greater than that of the traditional soymilk (1053). This result also support that a large number of hydrophobic regions were exposed to the surface of protein particles, which is consistent with the red shift of the tryptophan fluorescence spectrum.