Recently, different rapid methods with high sensitivity and
specificity have been developed to overcome the limitations of
conventional methods for the detection and identification of
foodborne pathogens. Furthermore, researchers are still developing
novel methods with improvements in terms of rapidity,
sensitivity, specificity and suitability for in situ analysis
and distinction of the viable cell (Zhao et al., 2014). Rapid
detection methods are important, particularly in food industry,
as they are able to detect the presence of pathogens
in raw and processed foods immediately. Rapid methods are
also sensitive enough to detect pathogens that present in low
numbers in the food. Sensitivity is important because a single
pathogen present in food has the risk to cause infection.
Rapid methods are more time-efficient, labor-saving and able to
reduce human errors (Mandal et al., 2011). Nevertheless, each
of the rapid method has its own advantages and limitations.
Generally, rapid detection methods are categorized into nucleic
acid-based, biosensor-based and immunological-based methods
(Zhao et al., 2014). This review examines these recent rapid
detection methods and their applications in foodborne bacterial
pathogens detection and along with their advantages and
limitations.
Recently, different rapid methods with high sensitivity andspecificity have been developed to overcome the limitations ofconventional methods for the detection and identification offoodborne pathogens. Furthermore, researchers are still developingnovel methods with improvements in terms of rapidity,sensitivity, specificity and suitability for in situ analysisand distinction of the viable cell (Zhao et al., 2014). Rapiddetection methods are important, particularly in food industry,as they are able to detect the presence of pathogensin raw and processed foods immediately. Rapid methods arealso sensitive enough to detect pathogens that present in lownumbers in the food. Sensitivity is important because a singlepathogen present in food has the risk to cause infection.Rapid methods are more time-efficient, labor-saving and able toreduce human errors (Mandal et al., 2011). Nevertheless, eachof the rapid method has its own advantages and limitations.Generally, rapid detection methods are categorized into nucleicacid-based, biosensor-based and immunological-based methods(Zhao et al., 2014). This review examines these recent rapiddetection methods and their applications in foodborne bacterialpathogens detection and along with their advantages andlimitations.
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