2.3. qRT-PCR
The hdcA primers and the housekeeping gene gyrB primers
(used for the control group) for qRT-PCR are shown in Table 1. The
temperature condition for the qRT-PCR was 42 C for 5 min, 95 C
for 10 s, followed by 40 cycles of 95 C for 5 s and 63 C for 30 s,
based on the results of our investigation.