Abstract
Low cell competency for regeneration and transformation is the main cause of so-called recalcitrance to transform a species or a genotype. A research was conducted to determine the optimum conditions for in vitro plant regeneration involving organogenesis in Citrus aurantium, which is an important rootstock worldwide. Seeds with peeled teguments were germinated in vitro, either kept in dark for 6 weeks or maintained in the absolute dark for 4 weeks followed by 10 days in 16-h photoperiod (56 μmol m-2 s-1) at 27 ± 2°C. Epicotyl-originated explants were cultured in MS medium supplemented with 6-benzylaminopurine (BAP) (0, 1, 2 and 3 mg L-1) and Naphthaleneacetic acid (NAA) (0, 0.05, 0.1 and 0.2 mg L-1) to