The NEFA concentrations were analyzed using a two-reaction,
enzymatic-based colorimetric assay from Wako (NEFA-C, Wako
Chemicals GmbH, Neuss, Germany), based on the ability of NEFA
to acylate coenzyme A in the presence of CoA synthetase. Glucose
was analyzed by the glucose oxidase method using the Glucose Infinity
kit from Sigma (Sigma Diagnostic Inc., St. Louis, MO, USA).