The effect of pullulan (PUL) on the retrogradation of rice starch (RS) was investigated by means of rapidvisco-analyzer (RVA), rotational rheometer, differential scanning calorimetry (DSC), and X-ray diffraction(XRD). RVA results showed that addition of pullulan significantly decreased the breakdown and setbackvalues, which meant that the short-term retrogradation of RS was inhibited. The dynamic time sweepof samples also proved the retarding effect of pullulan on the retrogradation of RS. DSC curves showedclearly that pullulan significantly reduced the retrogradation enthalpy of amylopectin, and the kineticsof retrogradation was analyzed using the Avrami model. XRD results showed that recrystallinity of RSwas reduced from 11.565% to 8.841% with the addition of pullulan and this was in line with the DSCresults. It could be concluded that the addition of pullulan apparently influenced not only the short-termretrogradation of amylose, but also the long-term retrogradation of amylopectin.