there are several colors of mulberry fruits, even from the same species, which may contain different amounts of anthocyanins as well as different antioxidant levels. It has been found that the extraction method of M. nigra fruits has a significant effect on its biological
activities, such as inhibition of haemolysis (Naderi et al., 2004). However, a study of anthocyanin levels and biological activities within a single species of mulberry fruits has never been reported. In addition, temperature and pH have an affect on color degradation
of mulberry fruit extract, which may be associated with degradation of anthocyanin (Suhl, Noh, Kang, Kim, & Lee, 2003). In order to successfully generate mulberry fruit product as a food
supplement, the effects of temperature and light need to be investigated.