produced 5.37 and 5.86% of bioethanol concentration, respectively. The concentration of bioethanol increased
as the amount of water was increased. However, different amount of water applied to the banana mash only gave
slight difference on the concentration of bioethanol when 15 to 35% amount of water was used. According to the
data, the values of the total soluble solid for the fermented banana mash were lower than before fermentation (Table 3). Among the fermented banana mash with different amount of water, fermentation of banana mash without water exhibited the higher value of total soluble solid, followed by fermentation with 15, 35 and 25% of water. From the pH results, measurements of the pH before fermentation were higher than after fermentation. After fermentation, fermented banana mash with 35% of water had the highest pH measurement among the others, followed by fermentation without water with 15 and 25% of water. Comparison of the bioethanol concentration of banana
mash using rotten and fresh banana fruit were shown in Figure 4. From the plotted graph, the highest concentration of bioethanol was produced from the fermentation that had been used rotten banana fruit with 5.79% (v/v), followed by fresh banana fruit with 4.12% (v/v) of bioethanol. Before fermentation, the values of total soluble solid of fresh banana fruit were higher than rotten banana fruit, same as after fermentation (Table 4). For both rotten and fresh banana fruit, the value of total soluble solid after fermentation were lower than after fermentation, as sugar content of banana mash was used by yeast to do the fermentation. From the pH measurement, after fermentation, fresh banana mash
exhibited the lower value than before fermentation. The concentration, total soluble solid and pH of bioethanol
can be considered as significantly difference at p < 0.05. The result of bioethanol produced from fermented
banana mash showed that, the concentration of bioethanol produced range from 5.84 to 7.08% (Figure 5).
The lowest volume of 5.84% was produced from the fermented banana mash treated with conventional method (without enzyme), while the highest volume of 7.08% (v/v) was produced from the fermented banana
mash treated with mixture of enzymes saccharification method. Banana mash treated with cellulase produce
6.64% (v/v) of bioethanol, while by using pectinase saccharification method, the result was 7.03% (v/v) of
bioethanol. The values of total soluble solid of banana mash were higher before fermentation than after fermentation (Table 5). Among the different enzyme saccharification method, after fermentation, the fermented banana mash treated with conventional method exhibited the highest value of total soluble solid. From the pH measurement, after
fermentation, fermented banana mash treated with the cellulase saccharification method exhibited the higher pH
value among the other saccharification method. The concentration of bioethanol, total soluble solid and pH values of bioethanol can be considered as significantly difference based on ANOVA method at p < 0.05