Most of the commercial proteases producing organisms are Bacillus sp. (Abo-Aba et al. 2006).
The potential of cow dung as a biomass substrate for the production of alkaline protease by using Bacillus sp. has not yet been completely exploited.
The main objective of the present study was the production of alkaline proteases by Bacillus subtilis utilizing cow dung as an energy source, and determination of the optimum conditions necessary for thefor the production of these enzymes