TLC of Lipids
The total lipids of mung bean samples were fractionated by
a reported method [19] using silica gel, G 60 Merck type 5721 and 20 cm 9 20 cm glass plates with 0.25 mm
thickness. The developing solvent system was n-hexane:
diethyl ether: acetic acid glacial (80: 20: 2, V/V/V). The
separated fractions of total lipids were visualized by
exposure to iodine vapor in a closed chamber after drying.
All lipid fractions were identified on thin layer plates by
comparing their Rf values with those of known lipid standards.
For quantitative analysis, the different lipid fractions
were scanned by using Shimadzu TLC-Scanner (C-S-910).
The area under each peak was measured by the triangulation
method [20].The percentage of each component was
calculated with regard to the total area as follows by a
reported procedure [21]
% Component ¼ Area of each peak=Total peaks area
100