There are some limitations to any PCR typing scheme. For instance, a
number of type 1 IncC plasmids contain a backbone deletion extending
to the right of ARI-A (Harmer and Hall, 2014), and for these the RH929/
RH930 PCR to detect the right hand junction of ARI-A may produce no
amplicon even though the RH930/RH339 PCR detecting the left hand
junction generates the expected amplicon. Similarly, deletions arising