2.5. Differential scanning calorimetry
Differential scanning calorimetry (DSC) thermographs were produced using a PerkineElmer DSC Pyris 1 (Perkin Elmer, Norwak,Connecticut, USA). Approximately 40 mg of each batter mix was weighed into stainless-steel pans capable of remaining intact up to medium pressures. An empty sealed pan was used as a reference.
The samples were scanned from 25 C to 130 C, and the heating rate was 10 C/min. DSC data were evaluated from the thermograms to calculate the transition temperatures, onset (To), peak (Tp)
and conclusion (Tc), and enthalpy (DH) using Perkin Elmer Pyris
Version 3.81 software. Measurements were performed in triplicate.