Considering the subcellular localization of the proteins
identified in each group from both approaches (biotinylation and purification of biotinylated
proteins), we observed that most of identified proteins which found only in granule-containing
hemocytes were annotated as secreted (externally-bound) proteins. Interestingly, alpha-2-
macroglobulin (A2M) showed the redundant protein identified and confirmed by these two
approaches.