In addition, this recombinant Omp31 extract formulated in IFA elicited serum antibodies capable of killing efficiently B.ovis in the presence of homologeous o heterologeous serum and induce a mucosal immune response. Also, as previously reported, ram specific antibodies elicited by the chimera BLSOmp31 emulsified in IFA were able to produce complement mediated bacteriolysis of B.ovis.