One of the constant challenges to nano-science community is to present cost–effective methods to fabricate nanostructures. Although conventional nanolithography techniques, such as electron beam lithography and focused ion beam lithography, are commercially available and extensively implemented in nanofabrication, they are time-consuming and expensive. Due to these limitations in conventional approaches, more recent fabrication techniques including soft lithography, nano-imprinting lithography and nanosphere lithography have been developed. In addition to these recent approaches, there are some simpler techniques to create regular arrays of nano-holes or nano-pillars, such as nanosphere photolithography (NSP). The NSP technique uses two-dimensional (2D) self-assembled arrays of nanometre-sized polystyrene (PS) spheres as a deposition mask over photoresist. Apart from growing interest into fabrication process, characterization of surface properties is part of the research interest in