The same reactivities of this serum were detected by a flow cytometric assay using isolated lymphocytes.
The serum became negative to the flow bead test after platelet absorption.
The results demonstrated that flow bead test was a sensitive and specific assay.
Dilution studies further proved the higher sensitivity of the flow bead test compared to the cytotoxicity test.
Many typing sera started to lose cytotoxic reactivity at a 1:10 dilution. However, the same specificity was still detected by the flow bead test at the same, or higher, serum dilution.