Hence, it has become imperative to establish a suitable protocol to generate enough materials to ensure its supply for pharmaceutical industries without further depopulating this species. Plant tissue culture techniques offer a viable tool for mass multiplication and conservation of rare & endangered medicinal plants for meeting the pharmaceutical needs3. Limited tissue culture work has been done on Psorelea corylifolia4,5. The present study explains an efficient in vitro method for rapid mass propagation of P. Corylifolia from various explants using different combination & concentrations of auxins & cytokinin.