Figure 4: The cell surface GRP78 regulates the activity and secretion of MMP-2 and EMT. (a) Gelatin zymography analysis of the activity of
MMP-2 and MMP-9 in Mahlavu and SMMC7721 cells treated with the N-20 antibody. (b) Western blot analysis of the secretion of MMP-2
and MMP-9 in Mahlavu and SMMC7721 cells treated with the N-20 antibody. (c) Gelatin zymography analysis of the activity and secretion
of MMP-2 in the ΔKDEL transfectants. (d)Western blot analysis of the expression of E-Cadherin and N-Cadherin in Mahlavu cells treated
with the N-20 antibody. (e)Western blot analysis of the expression of E-Cadherin and N-Cadherin in SMMC7721 cells treated with the N-20
antibody. (f) Western blot analysis of the expression of E-Cadherin and N-Cadherin in the ΔKDEL transfectants. UT, untreated. IgG goat
isotype IgG. N20, the N20 antibody.