The mother culture substrates were prepared using boiled corn seeds, sliced boiled sweet potato, and sliced boiled cassava in test bottles following the Table 2 formulation. The test bottles containing the media were sterilized in an autoclave for 15 minutes at 121oC and kept for 24 hours for cooling. A small amount of pure culture was inoculated aseptically and incubated in a dark place at an ambient temperature. The mycelium running of mother culture was observed daily [7], [8].