The method used for infection of potato tubers
with the fungus F. eumartii involves wounding of
the inoculated tissue, therefore, in order to determine
whether the expression of the StCyP gene is
at least partly associated with the response to
fungal infection and/or mechanical wounding
StCyP mRNA accumulation was compared in
both wounded tubers treated with sterile PDA
disk and in wounded plus infected potato tubers,
at different times after the onset of treatment (Fig.
5). The StCyP mRNA is barely detectable in
non-wound-non-inoculated tubers After wounding the level increases, reaching a
maximum at 24 h after wounding and then decreases
(Fig. 5A, dashed bars and Fig. 5B, W). By
72 h after wounding, StCyP mRNA accumulation
was close to that initially observed at 4 h after
wounding, the shortest time analysed here