(DPPH). Stock solutions of each extract (Table 1) were prepared
by dissolving 7.5 mg of the extract in 25 mL of ethanol. From these
stock solutions, work solutions were prepared at different masses
(1–40 lg mLÀ1) and dissolved in 3 mL of ethanol and then added
to an ethanolic solution of 0.1 mL of free radical DPPH (1 mM).
After 30 min incubation, spectra of the resultant solutions were
recorded in an Evolution 60 S spectrophotometer (Thermo Scien-
tific) with absorbance read at 517 nm [28]. Tests were carried
out in triplicate. The results are reported as lg mLÀ1 of 50%
inhibition (IC50) that is, mass of crude extract required to cause
50% inhibition of DPPH.