Two identical experiments, separated in time, with 1,200 eggs from a different flock of the same strain (Cobb-500) and age (38 wk old), were conducted. Broiler breeder eggs (Belgabroed, Merksplas, Belgium) were individually numbered and weighed before being evenly divided among 3 forced-draft incubators with a maximum capacity of 600 eggs (Pas Reform, Zeddam, the Netherlands), which were adjusted with a focus controller from Petersime NV (Zulte, Belgium). All eggs were stored for 3 to 4 d before incubation. The incubators were set at a standard dry bulb temperature of 37.6°C and a wet bulb temperature of 30°C. At the beginning of embryonic day (ED) 16, 3 experimental groups (low, control, high; 400 eggs/group) were created by redistributing the eggs so that each group contained a mixture of eggs from the 3 incubators. All 400 eggs/treatment were placed on 3 trays (133 to 134 eggs/tray) in the incubator. Eggs were turned more than 90° every hour during the whole incubation period, until ED 18.5.
After redistributing the eggs at the beginning of ED 16, the incubator temperature (dry bulb temperature) was set at 34.6°C (low), 37.6°C (control), or 40.6°C (high) and remained at this temperature continuously until ED 18.5. The wet bulb temperature was kept constant at 30°C. At ED 18.5, the eggs were transferred from the turning trays to hatching baskets, and all the eggs were incubated at the standard temperature of 37.6°C until the end of incubation. Average egg weights at setting were 63.45 ± 0.20 g (experiment 1) and 63.98 ± 0.19 g (experiment 2) for the low-temperature group, 63.30 ± 0.20 g (experiment 1) and 64.10 ± 0.18 g (experiment 2) for the control group, and 63.64 ± 0.20 g (experiment 1) and 63.92 ± 0.19 g (experiment 2) for the high-temperature group
Two identical experiments, separated in time, with 1,200 eggs from a different flock of the same strain (Cobb-500) and age (38 wk old), were conducted. Broiler breeder eggs (Belgabroed, Merksplas, Belgium) were individually numbered and weighed before being evenly divided among 3 forced-draft incubators with a maximum capacity of 600 eggs (Pas Reform, Zeddam, the Netherlands), which were adjusted with a focus controller from Petersime NV (Zulte, Belgium). All eggs were stored for 3 to 4 d before incubation. The incubators were set at a standard dry bulb temperature of 37.6°C and a wet bulb temperature of 30°C. At the beginning of embryonic day (ED) 16, 3 experimental groups (low, control, high; 400 eggs/group) were created by redistributing the eggs so that each group contained a mixture of eggs from the 3 incubators. All 400 eggs/treatment were placed on 3 trays (133 to 134 eggs/tray) in the incubator. Eggs were turned more than 90° every hour during the whole incubation period, until ED 18.5. หลังจาก redistributing ไข่ที่ ED 16, incubator อุณหภูมิ (อุณหภูมิหลอดแห้ง) ถูกตั้งที่ 34.6° C (ต่ำ), 37.6° C (ระบบควบคุม), หรือ 40.6° C (สูง) และยังคงอยู่ที่อุณหภูมินี้อย่างต่อเนื่องจนถึง ED 18.5 อุณหภูมิหลอดเปียกถูกเก็บค่าคงที่ที่ 30 องศาเซลเซียส ที่เอ็ด 18.5 ไข่ถูกโอนย้ายจากถาดเปิดไปฟักตะกร้า และไข่ทั้งหมดถูก incubated ที่อุณหภูมิมาตรฐาน 37.6° C จนถึงจุดสิ้นสุดของคณะทันตแพทยศาสตร์ น้ำหนักไข่เฉลี่ยที่ตั้งถูก 63.45 ± 0.20 g (ทดลอง 1) และ 63.98 ± 0.19 g (ทดสอบ 2) สำหรับกลุ่มอุณหภูมิต่ำ 63.30 ± 0.20 g (ทดลอง 1) และ 64.10 ± 0.18 กรัม (ทดลอง 2) สำหรับกลุ่มควบคุม และ 63.64 ± 0.20 g (ทดลอง 1) และ 63.92 ± 0.19 g (ทดสอบ 2) สำหรับกลุ่มอุณหภูมิสูง
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