A total of 20 samples from ewe milk were analyzed, of which 10 samples were collected from Myaneh (15 herds) and 10 samples obtained from Hashrood (15 herds) two cities in Azarbayjan-esharqi (north-west of Iran). In both places, samples were taken at 10 different days and collected from bulk milk tank. All samples were collected according to EN ISO 707:2001 in sterile bottles of 250 mL and transported to the laboratory under refrigeration (4 C) within 36 h