to 100 ml Inoculum medium contained Basal Medium and 1.0% lactose (Merck) as the carbon source. The medium was autoclaved at 115°C for 20min. Fermentation medium (enzyme production medium) contained Basal Medium and 1% cellulose (Sigma). The medium was autoclaved at 121°C for 20 min and pH was adjusted to 6.0.