Primers were designed using the Primer3 software (version 0.4.0; http://frodo.wi.mit.edu/primer3/). Primer specificity was confirmed by melting curve analyses of real-time RT-PCR products (81 cycles of 55°C for 30 s).
Primers were designed using the Primer3 software (version 0.4.0; http://frodo.wi.mit.edu/primer3/). Primer specificity was confirmed by melting curve analysesof real-time RT-PCR products (81 cycles of 55°C for 30 s).