The production of double haploids through androgenesis is used by breeders to produce homozygous lines
in a single generation. Androgenesis can be achieved by isolated microspore culture, which, however,
allows the production of embryogenesis with a very low efficiency. In order to improve the overall embryogenesis
in pepper, we study the differences of microspore embryogenesis in different genotypes of
pepper, and also document the effect of growth regulators in pretreatment media, and activated charcoal
(AC) on embryogenesis induction. Fifty different pepper genotypes were evaluated, and the swollen rate
of microspores from different genotypes varied from 3.11% to 29.56% with the mean value of 13.13%.
Microspores from genotype ‘36’ had the highest swollen rate, and the lowest swollen rate of microspores
was observed in genotype ‘26’. It was concluded from the statistical results of L9 (33) orthogonal test that
changes in the level of BA influenced the swollen rate of microsporesmore significantly, and the combination
of 0 mg∙l−1 6-benzyladenine (BA), 0.2 mg∙l−1 α-naphthaleneacetic acid (NAA) and 0.5 mg∙l−1
kinetinin (Kin) was best. AC at a concentration of 0.05% could act as a promoter of embryogenesis in the
microspore culture of different pepper genotypes, while the more significant effect was observed with
the low responsive genotypes.