The contents of 20 fluconazole capsules / uncoated
tablets / dispersible tablets were taken and powdered. The
powder equivalent to 25 mg of fluconazole was
accurately weighed and transferred into a 50 ml
volumetric flask. To this, 30 ml of water was added and
sonicated for 10 min with occasional shaking to disperse
and dissolve the contents. The volume was made up to 50
ml with water to give 500 μg/ml of fluconazole solution.
This solution was filtered through 0.45 μ membrane filter
and diluted suitably using mobile phase to obtain 40
μg/ml solution.