Seeds were sown on an a petri dish comprising half-strength macroelements and full-strength microelements of MS medium (Murashige and Skoog, 1962) containing 0.7% Bacto agar (Difco Laboratories, Detroit, MI), 2% sucrose, and 0.1% activated charcoal. The pH value of the medium was adjusted to 6 prior to autoclaving at 121◦C of 1.05kg/cm2 for 20min. The seeds were incubated
at 25 ± 1◦C under a 14-h photoperiod at an irradiance level of 40molm−2 s−1 provided by daylight cool white fluorescent light-
ing.
To determine viability, seeds were incubated in 1% (w/v) TTC solution for 1 d at 25◦C in the dark. The percentage of viable TTCstained embryo seeds was calculated as the viability rate of seeds.
Seeds were sown on an a petri dish comprising half-strength macroelements and full-strength microelements of MS medium (Murashige and Skoog, 1962) containing 0.7% Bacto agar (Difco Laboratories, Detroit, MI), 2% sucrose, and 0.1% activated charcoal. The pH value of the medium was adjusted to 6 prior to autoclaving at 121◦C of 1.05kg/cm2 for 20min. The seeds were incubated at 25 ± 1◦C under a 14-h photoperiod at an irradiance level of 40molm−2 s−1 provided by daylight cool white fluorescent light- ing.To determine viability, seeds were incubated in 1% (w/v) TTC solution for 1 d at 25◦C in the dark. The percentage of viable TTCstained embryo seeds was calculated as the viability rate of seeds.
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