Before the fixed plant materials were loaded onto the one-step
sample preparation column for ABA extraction and purification, the
oven or microwave dried materials were ground in a 2-mL plastic
centrifuge tube with a plastic grinding rod, while the materials fixed
by boiling or Carnoy’s fixative were ground using a mortar and a
pestle. And the samples fixed by Carnoy’s fixative were diluted by
adding equal volume of water to make its ethanol concentration
lower than 40% before loading to the column. The oven drying fixation
method was used in this study for the detection of ABA in
peanut plants during drought stress