In this work, we synthesized graphene oxide functionalized a surface-imprinted polymer based on the self-polymerization of dopamine to generate the imprinted cavity. Using minocycline as the template molecule,effects of experimental conditions in the imprinting procedure such as g-MAPS@GO percentage, templateconcentration, and self-polymerization time of dopamine on the selectivity and performance of theprepared molecularly imprinted polymers were investigated. The characteristics of the prepared surface-imprinted polymer were determined by scanning electron microscope, transmission electronmicroscope, energy dispersive spectroscopy, Fourier-transformed infrared spectra, X-ray photoelectronspectroscopy, thermal gravimetric analysis, atomic force microscope, and water contact angle. Theprepared graphene oxide functionalized surface-imprinted polymer showed good recognition capacityand enrichment performance for tetracycline antibiotics and was successfully applied to the detection ofthe target analytes in milk samples