Lipid peroxidation in plasma was measured by determining the concentration of MDA by
HPLC as described earlier [12].
For the determination of phospholipid fatty acids, 1 ml of plasma was immediately mixed
with 10 l of 5% butylated hydroxytoluene in ethanol and then stored at –70°C until assayed.
Plasma lipids were extracted with chloroform/methanol as described [13]. The phospholipids
were separated by thin-layer chromatography and after transmethylation, the fatty acid
methyl esters were separated by gas-liquid chromatography. The results are given as area
percentage of all 22 fatty acids detected.