The effect of different working concentrations was
considered in order to standardize the initial semen dilution.
The need to optimize this step was related to the large
variability in sperm concentration found in the ejaculates
collected from Mericanel della Brianza birds [23]. Furthermore,
the sperm concentration measured in fresh semen
sampleswas reported to affect the quality of frozen/thawed
turkey semen pellets [25]. The use of accurate working
concentrations instead of dilution rates is commonly used
in cryopreservation procedures for mammalian semen