Among myxosporidians, the two genera, Myxobolus and Thelohanellus have been recorded as the most common in aquaculture (Sarkar and Raychaudhuri, 1986; Adriano et al., 2009). In the present study, T. bifurcata Basu and Haldar, 1999 has been recorded for the first time from a fish pond in Punjab (earlier was reported from Nanaghat, District Nadia, West Bengal)