Therefore, a filtersteriled
hydrolysate produce from pineapple peel under optimal condition was used as fermentation
medium with no nutrient supplementation. Afterward free and immobilized cell of S. cerevisiae and E.
aerogenes with a loading of 20% (w/v) was operated under anaerobic condition and a temperature
controlled at 30oC. The samples were collected every 6 h for 4 days (96 h) to determined sugar
concentration and the production of ethanol and hydrogen.