The parameters observed in this experiment were: (1) total fresh weight (FW) of PLBs; (2) average of FW of PLBs added per subculture period (g); (3) total number of PLBs; (4) average number of PLBsadded per subculture period; and (5) percentage of PLB browning(%). Observations were made daily to monitor changes in culture through out the incubation period. Data was recorded every monthof growth and PLB proliferation.