Carbohydrate and citrate metabolism in the fermenting cocoa pulp-bean mass. Enzymatic reactions carried out by members of the Lactobacillaceae are depicted in blue,
enzymatic reactions carried out by members of the Enterobacteriaceae are depicted in red, and enzymatic reactions that can be carried out by both families are depicted in black.
Enzymatic reactions carried out by members of the Acetobacteraceae are depicted by green reaction numbers. A: sucrose-PTS transporter (scrA); B: fructose/mannitol-PTS transporter
(fruA); C. glucose-PTS transporter (ccr); D. GPH family glycoside-pentoside-hexuronide:cation symporter (lacS); E: 2HCT-family citrate/acetate antiporter (citW); F: CitMHSfamily
citrate transporter (citM); G: GntP-family gluconate:Hþ-family symporter (gntP) H: uncharacterized di/tricarboxylate transporter; 1: glucose 6-phosphate isomerase (pgi); 2:
mannitol 1-phosphate 5-dehydrogenase (mtlD); 3: 6-phosphofructokinase (pfkA); 4: fructose-bisphosphate aldolase (fba); 5: triosephosphate isomerase (tpiA); 6: glyceraldehyde 3-
phosphate dehydrogenase (gapAB); 7: phosphoglycerate kinase (pgk); 8: phosphoglycerate mutase (gpmA or gpmB); 9: enolase (eno); 10: pyruvate kinase; (pyk); 11: L-lactate
dehydrogenase (ldh); 12: D-lactate dehydrogenase (ldh); 13: glucokinase (glk); 14: glucose 6-phosphate dehydrogenase (zwf); 15: 6-phosphogluconolactonase (pgl); 16: 6-
phosphogluconate dehydrogenase (gnd); 17: ribulose-phosphate epimerase (rpe); 18: phosphoketolase (xpk); 19: acetate kinase (ackA); 20: phosphate acetyltransferase (pta);
21: acetaldehyde dehydrogenase (adhE); 22: NAD(P)-dependent alcohol dehydrogenase (adh); 23: fructokinase (cscK); 24: arabitol/mannitol dehydrogenase (dalD); 25: a-glucosidase
(agl); 26: b-fructofuranosidase (scrB); 27: methylglyoxal synthase (mgsA); 28: lactoylglutathione lyase (gloA); 29: hydroxyacylglutathione hydrolase (gloB); 30: pyruvate
carboxylase (pyc); 31: phosphoenolpyruvate carboxykinase (pckA); 32: fructose 1,6-bisphosphatase (fbp, glpX); 33: phosphoenolpyruvate synthase (pps); 34: acetyl-CoA synthetase
(acn); 35: NADþ-dependent aldehyde dehydrogenase (dhaS); 36: phosphoenolpyruvate carboxylase (ppc); 37: citrate lyase (citGXFEDC); 38: NADþ-dependent oxaloacetatedecarboxylating
malate dehydrogenase (sfcA); 39: NADþ/FAD-dependent malate dehydrogenase (mdh); 40: fumarase (fumA/fumB/fumC); 41: fumarate reductases (sdhABCD); 42:
TPP-dependent pyruvate decarboxylase; 43: a-acetolactate synthetase (ilvHI); 44: a-acetolactate decarboxylase (budA/aldB); 45: acetoin reductases (budC/butA); 46: pyruvateformate
lyase (pfl); 47: pyruvate dehydrogenase complex (pdh); 48: pyruvate oxidase (cidC).